Cell transformation as aberrant differentiation: Environmentslly dependent spontaneous transformation of NIH 3T3 cells
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منابع مشابه
Overexpression Transformation of NIH/3T3 Cells by Ornithine Decarboxylase
Ornithine decarboxylase (ODO plays a rate-limiting role in polyamine biosynthesis and is intimately associated with cell proliferation and func tion. Although elevated levels of ODC mRNA, protein, and enzyme activity are consistently detected in transformed cells and tumors, the question remains as to whether ODC gene overexpression has a causative role in tumorigenesis. We have stably transfec...
متن کاملTransformation of NIH/3T3 cells by ornithine decarboxylase overexpression.
Ornithine decarboxylase (ODC) plays a rate-limiting role in polyamine biosynthesis and is intimately associated with cell proliferation and function. Although elevated levels of ODC mRNA, protein, and enzyme activity are consistently detected in transformed cells and tumors, the question remains as to whether ODC gene overexpression has a causative role in tumorigenesis. We have stably transfec...
متن کاملEffect of adeno-associated virus on transformation of NIH 3T3 cells by ras gene and on tumorigenicity of an NIH 3T3 transformed cell line.
Transfection of NIH-3T3 cells with the plasmid pJ234, containing DNA from the human bladder carcinoma T24 cell line (ras gene), results in their transformation. Adeno-associated virus did not affect significantly the number of the transformed foci when different multiplicities of infection were used and when the virus was added to the cultures at different time intervals before or after transfe...
متن کاملOverexpression of the c-src protein does not induce transformation of NIH 3T3 cells.
NIH 3T3 mouse cells were transfected with plasmids that induce efficient expression of either (i) the Rous sarcoma virus v-src gene, (ii) the chicken c-src gene, or (iii) a recombinant gene combining the 5' portion of c-src with the 3' end of v-src. Focus formation in tissue culture and formation of large colonies in soft agar did not occur in cells transfected with c-src. Cells transfected wit...
متن کاملSignal transducers and activators of transcription 5B potentiates v-Src-mediated transformation of NIH-3T3 cells.
Previously, we reported that whereas both signal transducers and activators of transcription (STAT) 5A and STAT5B can be activated with respect to tyrosine phosphorylation and DNA binding potential by Src kinase, only STAT5B was translocated to the nucleus, where it presumably activates unique downstream responses. To help elucidate the functional consequences of STAT5B activation by v-src, the...
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ژورنال
عنوان ژورنال: Cell Research
سال: 1990
ISSN: 1001-0602,1748-7838
DOI: 10.1038/cr.1990.20